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Image Search Results
Journal: Microorganisms
Article Title: Designing a Multiplex PCR-xMAP Assay for the Detection and Differentiation of African Horse Sickness Virus, Serotypes 1–9
doi: 10.3390/microorganisms12050932
Figure Lengend Snippet: Schematic representation of multiplex PCR-xMAP workflow. Nucleic acid is extracted in an automated extraction robot (i). The resulting eluent is then split across two wells and combined with biotinylated primers from multiplex reaction mix A in one well (for the detection of AHSV serotypes -1, -3, -5, and -7) and multiplex reaction mix B in the second well (for the detection of AHSV serotypes -2, -4, -6, -8, and -9) (ii). This mixture is then denatured, followed by the addition of qPCR mastermix (iii) and then RT-PCR (iv). The resulting amplified product is added to a 96-well plate preloaded with microspheres (v). Hybridisation of amplified product (if present) to microspheres occurs during an incubation step at 50 °C. Streptavidin, R-Phycoerythrin Conjugate (SAPE) is then added to each well and binds to the amplified product (if present) that is attached to the microsphere (vi). Finally, detection of both SAPE and microsphere is performed using the Bio-Plex 200 instrument (vii). Schematic created with Biorender.com.
Article Snippet: The plate was then placed in a
Techniques: Multiplex Assay, Extraction, Reverse Transcription Polymerase Chain Reaction, Amplification, Hybridization, Incubation
Journal: bioRxiv
Article Title: A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex ® technology (Bio-Plex ® 200 or MAGPIX ® )
doi: 10.1101/2020.08.10.243980
Figure Lengend Snippet: IgG antibody levels (RAU) measured against 19 P. vivax proteins in samples from malaria-endemic areas, using either non-magnetic or magnetic beads and run on a Bio-Plex ® 200 instrument. *** p<0.001, **** p<0.0001.
Article Snippet: Non-magnetic beads can only be run on flow-based instruments such as the
Techniques: Magnetic Beads
Journal: bioRxiv
Article Title: A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex ® technology (Bio-Plex ® 200 or MAGPIX ® )
doi: 10.1101/2020.08.10.243980
Figure Lengend Snippet: IgG antibody levels (RAU) measured against 19 P. vivax proteins in samples from malaria-endemic areas, using magnetic beads and run on either a Bio-Plex ® 200 instrument or MAGPIX ® instrument. **** p<0.0001.
Article Snippet: Non-magnetic beads can only be run on flow-based instruments such as the
Techniques: Magnetic Beads
Journal: bioRxiv
Article Title: A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex ® technology (Bio-Plex ® 200 or MAGPIX ® )
doi: 10.1101/2020.08.10.243980
Figure Lengend Snippet: IgG antibody levels (RAU) measured against 19 P. vivax proteins in samples from malaria-endemic areas, using non-magnetic beads and run on a Bio-Plex ® 200 compared to use of magnetic beads run on a MAGPIX ® instrument. **** p<0.0001.
Article Snippet: Non-magnetic beads can only be run on flow-based instruments such as the
Techniques: Magnetic Beads